Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli
β Scribed by Steven J. Beard; Rohani Hashim; Guanghui Wu; Marie R.B. Binet; Martin N. Hughes; Robert K. Poole
- Book ID
- 117516272
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 213 KB
- Volume
- 184
- Category
- Article
- ISSN
- 0378-1097
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The pit+ gene, encoding the phosphate (inorganic) transport system of Escherichia coli, was isolated from a library of E. coli genes inserted in the cosmid vector pHC79. A 25.5-kb chromosomal DNA fragment was shown also to carry the gor locus encoding glutathione oxidoreductase. Physical mapping pla
An inorganic phosphate transport mutant has been isolated as a sn-glycerol-3-phosphate auxotroph and characterized genetically. Two lesions are responsible for the transport defect. One lesion, pst, is located at minute 74 of the E. coli genetic map while the other lesion, pit, is located at minute