The pit+ gene, encoding the phosphate (inorganic) transport system of Escherichia coli, was isolated from a library of E. coli genes inserted in the cosmid vector pHC79. A 25.5-kb chromosomal DNA fragment was shown also to carry the gor locus encoding glutathione oxidoreductase. Physical mapping pla
Evidence for the transport of zinc(II) ions via the Pit inorganic phosphate transport system in Escherichia coli
β Scribed by Steven J. Beard; Rohani Hashim; Guanghui Wu; Marie R.B. Binet; Martin N. Hughes; Robert K. Poole
- Book ID
- 109324351
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 211 KB
- Volume
- 184
- Category
- Article
- ISSN
- 0378-1097
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The ugp structural genes, coding for the pho regulon dependent sn-glycerol-3-phosphate transport system, were cloned in pBR322 and characterized. The expression of the cloned ugp system was phoB dependent. Cells containing the ugp plasmid overproduced the G3P binding protein upon phosphate starvatio