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Evidence for mutations in the structural gene for homocitrate synthase in Saccharomycopsis lipolytica

โœ Scribed by Gaillardin, Claude ;Heslot, Henri

Book ID
104693479
Publisher
Springer
Year
1979
Tongue
English
Weight
651 KB
Volume
172
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

Eight strains devoid of homocitrate synthase activity were found among lysine requiring mutants of the yeast Saccharomycopsis lipolytica. Genetic analysis of these strains showed that they were all affected at the same locus LYS 1. Three lines of evidence suggest that this locus defines a structural gene for homocitrate synthase. First, the mutations show various degrees of intragenic complementation; it could be shown in some cases that the hybrid enzyme formed in vivo displayed modified properties in vitro. Second, reversion of some of these mutations can result in a modified enzyme (desensitized). Third, a feedback mutant of homocitrate synthase was directly isolated from the wild type strain, and shown to carry a single mutation at of near LYS 1. We also present here the first attempts at genetic fine mapping in Saccharomycopsis lipolytica.

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