Multidrug-resistant human neuroblastoma cell lines obtained by selection with vincristine or actinomycin D from two independent clonal lines, SH-SY5Y and MC-IXC, have 3- to 30-fold more cell surface epidermal growth factor (EGF) receptors than the drug-sensitive parental cells as indicated by EGF bi
Evidence for increased epidermal growth factor receptors in human sarcomas
β Scribed by Gerard Cowley; Jeffrey McIlhinney; Barry Gusterson; Bradford Ozanne; Cyril Fisher; Brian Reeves
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- French
- Weight
- 909 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0020-7136
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The development of a radioimmunoassay (RIA) for the human epidermal growth factor receptor solubilized with nonionic detergents which employs iodinated epidermal growth factor (125I-EGF) as the specific ligand is described. A monoclonal antibody (R1) that binds specifically to human EGF receptors [W
## Abstract Previous studies have shown that epidermal growth factor (EGF) stimulates bone resorption in organ culture via a prostaglandinβmediated pathway, and that there are specific receptors for EGF on mouse bone (Tashjian and Levine, β²78; Shupnik et al., β²80). The present study demonstrates th
## Abstract Between 60% and 100% of epidermal growth factor (EGF) binding activity was recovered from membranes of the A431 human epidermoid carcinoma cell line treated with solutions containing the nonionic detergent Triton Xβ100. Approximately half of the recovered binding activity was sedimented