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Evidence for electrically induced partial strand separation of DNA

✍ Scribed by M. Pollak; H. A. Glick

Publisher: Wiley (John Wiley & Sons)
Year: 1977
Tongue: English
Weight: 348 KB
Volume: 16
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1977.360160505

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✦ Synopsis

Abstract

We measured electrically induced changes of some optical properties of DNA solutions. These were the absorption coefficient and the reduced dichroism. The electric fields were applied in the form of 50–300‐μsec‐long pulses of varying field strength, up to 20 kV/cm. Changes of the optical properties were studied during the pulse. Solutions of varying DNA and salt concentrations were used. The qualitative predictions expected from the theory of electrically induced partial denaturation of the DNA were all observed. These are: (1) a decrease of the reduced dichroism at high fields; (2) a concurrent increase in the absorption coefficient; (3) a critical field above which effect (1) and (2) begin to occur; (4) a decrease of the magnitude of the above effects with increasing salt concentration; (5) a return to the status quo ante after short pulses.

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