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Evidence for a plasma-membrane-bound nitrate reductase involved in nitrate uptake ofChlorella sorokiniana

✍ Scribed by R. Tischner; M. R. Ward; R. C. Huffaker

Book ID: 104753101
Publisher: Springer-Verlag
Year: 1989
Tongue: English
Weight: 671 KB
Volume: 178
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00392522

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✦ Synopsis

Anti-nitrate-reductase (NR) immunoglobulin-G (IgG) fragments inhibited nitrate uptake into Chlorella cells but had no affect on nitrite uptake. Intact anti-NR serum and preimmune IgG fragments had no affect on nitrate uptake. Membrane-associated NR was detected in plasma-membrane (PM) fractions isolated by aqueous two-phase partitioning. The PM-associated NR was not removed by sonicating PM vesicles in 500 mM NaCl and 1 mM ethylenediaminetetraacetic acid and represented up to 0.8% of the total Chlorella NR activity. The PM NR was solubilized by Triton X-100 and inactivated by Chlorella NR antiserum. Plasma-membrane NR was present in ammonium-grown Chlorella cells that completely lacked soluble NR activity. The subunit sizes of the PM and soluble NRs were 60 and 95 kDa, respectively, as determined by sodium-dodecyl-sulfate electrophoresis and western blotting.

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