Glycosyl-phosphatidylinositol-anchored proteins exist in the plasma membrane ofChlorella saccharophila(Krüger) Nadson: Plasma-membrane-bound nitrate reductase as an example
✍ Scribed by Christine Stöhr; Frank Schuler; Rudolf Tischner
- Publisher
- Springer-Verlag
- Year
- 1995
- Tongue
- English
- Weight
- 395 KB
- Volume
- 196
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Experiments with plasma-membrane vesicles were performed in order to identify the attachment of hydrophobic nitrate reductase at the plasma membrane of Chlorella saccharophila. The enzyme was successfully removed from the plasma membrane with phosphoinositol-specific phospholipase C, and showed cross-reactivity with a monoclonal antibody (clone aGPI-3) raised against the glycosyl-phosphatidylinositol (GPI) anchor of Trypanosoma variant surface protein. The enzyme was labelled in vivo by feeding [3H]ethanolamine to the cells and underwent an hydrophobicity shift after treatment with phosphoinositol-specific phospholipase C. The attachment of this form of nitrate reductase to the plasma membrane via a GPI anchor was demonstrated.