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Evidence for 100% 13C NMR visibility of glucose in human skeletal muscle

✍ Scribed by Ronan Roussel; Pierre G. Carlier; Claire Wary; Gilberto Velho; Gilles Bloch

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
478 KB
Volume
37
Category
Article
ISSN
0740-3194

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✦ Synopsis

Abstract

The accuracy of the measurement of total muscle glucose by in vivo ^13^C NMR spectroscopy was tested in five normal volunteers during a euglycemic [1‐^13^C]glucose infusion. The NMR visible concentration calibrated using an external reference was compared with that calculated from plasma glucose concentration, assuming that glucose remained extracellular. The NMR measurement always provided higher values than the calculation from plasma glucose: 0.51 Β± 0.035 (mean Β± SE) versus 0.38 Β± 0.005 mmol/liter of muscle on average. This systematic difference was interpreted as reflecting the presence of muscle glucose‐6‐phospnate, co‐resonating with free glucose. Thus, glucose appeared to be virtually 100% NMR visible in human skeletal muscle.

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