Evidence based strategy for normalization of quantitative PCR data, in forensic miRNA-analysis
β Scribed by Sauer, Eva; Madea, Burkhard; Courts, Cornelius
- Book ID
- 122131665
- Publisher
- Elsevier
- Year
- 2013
- Tongue
- English
- Weight
- 198 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1875-1768
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## Abstract Careful validation of reference genes used for the normalization of realβtime RTβPCR data is required to obtain accurate results regarding gene expression. We evaluated the stability of seven commonly used reference genes in the cerebral cortex and hippocampus of rats 3 days following t
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become the preferred technique for studying low-abundance RNA expression. Proper normalization is a critical but often underappreciated aspect of quantitative gene expression analysis; popular endogenous control genes are usu