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Evidence against autorepression of the ββ′ operon in Escherichia coli

✍ Scribed by Tittawella, Ivor P. B.

Publisher
Springer
Year
1976
Tongue
English
Weight
380 KB
Volume
145
Category
Article
ISSN
0026-8925

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✦ Synopsis

By using rifampicin to increase the rate of beta and beta' synthesis in a heterodiploid strain of E. coli carrying the mutation rifpr (Km7), which codes for a rifampicin sensitive RNA polymerase to which the drug binds weakly, and the dominant mutation rifRD, which codes for a rifampicin resistant RNA polymerase, the concentration of these subunits in the cell was increased 1.6 fold. Measurements made after removal of rifampicin from the cells showed that the excess beta and beta' subunits did not reduce the rate of their own synthesis below normal.

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