The optimal parameters in the use of nuclease S1 in DNA reassociation kinetics in the presence of formamide have been determined. The conditions are especially suitable for the study of DNA rich in mole percent GC. A 10-fold dilution of the reassociation samples leading to a decrease in both NaCl an
Evaluation of the use of S1 nuclease to detect small length variations in genomic DNA
β Scribed by Anthony J. BROOKES; Ellen SOLOMON
- Book ID
- 115126404
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 885 KB
- Volume
- 183
- Category
- Article
- ISSN
- 1432-1327
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