Evaluation of the use of phase-specific gene promoters for the expression of enological enzymes in an industrial wine yeast strain

✍ Scribed by S. Puig; A. Querol; D. Ramón; J. E. Pérez-Ortín

• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 358 KB
• Volume: 18
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00154615

✦ Synopsis

Genes as POTI, HSPI04 and SSA3, which are late expressed in laboratory culture conditions are expressed only during the first few days in microvinifications in wine yeast cells. This effect is probably due to the different growth conditions and leads to useless levels of enzyme activity for a reporter gene. However the ACT1 promoter, which is constitutively expressed in laboratory conditions, produces sufficient amounts of enzyme activity in late fermentation phases.