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Evaluation of the interferon-γ ELISPOT-assay for quantification of peptide specific T lymphocytes from peripheral blood

✍ Scribed by Alexander Schmittel; Ulrich Keilholz; Carmen Scheibenbogen

Book ID
114051906
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
160 KB
Volume
210
Category
Article
ISSN
0022-1759

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Blood lymphocytes from HLA-A\*0201-subtyped melanoma patients and healthy controls were screened for the presence of T cells specific for HLA-A\*0201-binding melanoma and viral peptide antigens by the enzyme-linked immunoSPOT (ELISPOT) assay. CD8(+) cells were tested for peptide-specific IFN-gamma r