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Evaluation of the hydroxyproline isomers in blood serum

✍ Scribed by Georges H. Bellon; Alain Malgras; François Chastang; Jacques P. Borel

Book ID
115824621
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
357 KB
Volume
143
Category
Article
ISSN
0009-8981

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A new sensitive method for the quantitat
A new sensitive method for the quantitative evaluation of the hydroxyproline isomers
✍ Amin Bisker; Viviane Pailler; Alain Randoux; Jacques Paul Borel 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 720 KB

Proline, 4-hydroxyproline, and 3-hydroxyproline are combined with 7-chloro-4-nitrobenzo-2-oxa-I ,3-diazole and the fluorescent derivatives separated by thin-layer chromatography on silica gel prior to spectrofluorometric determination. The method is sensitive to 1 pmol, reproducible, and fast. It al