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A new sensitive method for the quantitative evaluation of the hydroxyproline isomers

โœ Scribed by Amin Bisker; Viviane Pailler; Alain Randoux; Jacques Paul Borel

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
720 KB
Volume
122
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Proline, 4-hydroxyproline, and 3-hydroxyproline are combined with 7-chloro-4-nitrobenzo-2-oxa-I ,3-diazole and the fluorescent derivatives separated by thin-layer chromatography on silica gel prior to spectrofluorometric determination. The method is sensitive to 1 pmol, reproducible, and fast. It also permits the measurement of the specific activities of proline and the hydroxyprolines.

' Abbreviation used: NBD-chloride, 7-chloro-4-nitrobenzo-2-oxa-l,3-diazole.

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