Sensitive spectrophotometric method for the quantitative estimation of collagen

✍ Scribed by Marcello Marotta; Guglielmo Martino

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 339 KB
• Volume: 150
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90443-9

✦ Synopsis

A method to estimate collagen concentration is described. It is based on the ability of the dye Sirius Red F3BA to quantitatively precipitate collagen in acetic acid solution. Collagen content is determined by evaluating spectrophotometrically the absorbance decrease of a Sirius Red/acetic acid solution. The assay can be used in the range from 1 to 50 pg protein. The method is specific, sensitive, simple, and rapid. 0 1985 Academic PBS, h.