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Evaluation of the direct agglutination test based on freeze-dried Leishmania donovani promastigotes for the serodiagnosis of visceral leishmaniasis in Sudanese patients

โœ Scribed by Khalid A. A. Abdallah; Bakri Y. M. Nour; Henk D. F. H. Schallig; Adil Mergani; Zohal Hamid; Abdallah Abd Elkarim; Osman K. Saeed; Ahmed A. Mohamadani


Book ID
110869608
Publisher
John Wiley and Sons
Year
2004
Tongue
French
Weight
75 KB
Volume
9
Category
Article
ISSN
1360-2276

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The performance of the direct agglutination test (DAT) was evaluated under field conditions in an endemic area of visceral leishmaniasis in eastern Sudan, using aqueous (Aq) antigen which has to be kept refrigerated and a newly developed freeze-dried (FD) antigen which is stable at ambient temperatu

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Tests on a panel of 42 1 serum samples from Ethiopia and 11 from Europe with the direct agglutination test (DAT) and freeze-dried antigen (Leishmania donovani 1 S) showed that a cut-off titre of 1:200 or 1:400 could be employed. Using a cut-off tiue of 1:400, 18 1 (883%) of 205 serum samples from vi