Evaluation of the direct agglutination test (DAT) using freeze-dried antigen for the detection of anti-Leishmania antibodies in stored sera from various patient groups in Ethiopia
✍ Scribed by L. Oskam; J.L. Nieuwenhuijs; A. Hailu
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 370 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0035-9203
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✦ Synopsis
Tests on a panel of 42 1 serum samples from Ethiopia and 11 from Europe with the direct agglutination test (DAT) and freeze-dried antigen (Leishmania donovani 1 S) showed that a cut-off titre of 1:200 or 1:400 could be employed. Using a cut-off tiue of 1:400, 18 1 (883%) of 205 serum samples from visceral leishmaniasis (VL) patients tested positive, as did 5 (13.9%) of 36 sera from patients with localized cutaneous leishmaniasis, 5 (71.4%) sera from 7 patients with diffuse cutaneous leishmaniasis and 8 (89.9%) sera from 9 HIV-positive VLpatients. We also found that 19 (57.6%) sera from 33 treated VL patients were still positive in the DAT at l-5 to 5 years after treatment.