Abstract
White rot fungus, Phanerochaete chrysosporium (P. chrysosporium), is oxidizing persistent organic pollutants (POPs) including dyes. The approaches of amplified fragment length polymorphism (AFLP) analysis and transmission electron microscope (TEM) observation were carried out to evaluate DNA and cell damaged by dyestuff and NaCl of different concentrations on P. chrysosporium. According to the data of P. chrysosporium cultivation and TEM observation, the damage of NaCl on P. chrysosporium was more significant than dyestuff. In addition, UPGMA method was performed to analyze the damage on P. chrysosporium by different concentration of NaCl and dyestuff by MVSP (Version 3.1). It showed that the DNA similarity between No. 2 (100 mg/L dyestuff and 15% NaCl) and No. 3 (100 mg/L dyestuff and 8% NaCl) was 66.7% when the concentrations of dyestuff was 100 mg/L. The DNA similarity between No. 3 (100 mg/L dyestuff and 8% NaCl) and No. 4 (300 mg/L dyestuff and 8% NaCl) was 88.9% when NaCl was kept at 8%, which was higher than 66.7%. No. 5 (300 mg/L dyestuff and 15% NaCl) was the most serious group. These results showed the damage by NaCl was significantly serious than that of dyestuffs. Therefore, the concentrations of NaCl in real dyestuff wastewater should be kept within 8%, keeping the treatment efficiency. ยฉ 2010 American Institute of Chemical Engineers Environ Prog, 2010