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Evaluation of the conformational equilibrium of reduced hen egg lysozyme by antibodies to the native form

โœ Scribed by Masayuki Oda; Aki Kitai; Akikazu Murakami; Miyuki Nishimura; Takatoshi Ohkuri; Yoshito Abe; Tadashi Ueda; Haruki Nakamura; Takachika Azuma

Book ID
116178541
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
757 KB
Volume
494
Category
Article
ISSN
0003-9861

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Separation of the tryptic peptides from
Separation of the tryptic peptides from reduced, alkylated hen egg white lysozyme by high-performance liquid chromatography
โœ L. Haeffner-Gormley; N.H. Poludniak; D.B. Wetlaufer ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 812 KB

We describe high-performance liquid chromatographic systems for the separation of the tryptic peptides of reduced, alkylated hen eggwhite lysozyme. The resolved peptides which contained 3-23 amino acid residues were identified by determinytion of amino acid composition. Gradients of acetonitrile wi