✦ LIBER ✦

Separation of the tryptic peptides from reduced, alkylated hen egg white lysozyme by high-performance liquid chromatography

✍ Scribed by L. Haeffner-Gormley; N.H. Poludniak; D.B. Wetlaufer

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 812 KB
Volume: 214
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)98523-6

No coin nor oath required. For personal study only.

✦ Synopsis

We describe high-performance liquid chromatographic systems for the separation of the tryptic peptides of reduced, alkylated hen eggwhite lysozyme. The resolved peptides which contained 3-23 amino acid residues were identified by determinytion of amino acid composition.

Gradients of acetonitrile with aqueous ammonium acetate or ammonium chloride were employed to elute from a reversed-phase C,, column, monitoring absorbance at 205 nm. 2 12 nm or 280 nm. Peptides containing Scarboxymethyl-cysteine eluted more rapidly than the corresponding S-ethylsuccinimido peptides.

📜 SIMILAR VOLUMES

Separation and characterization of the t

Separation and characterization of the tryptic peptide mapping of recombinant bovine growth hormone by reversed-phase high-performance liquid chromatography electrospray mass spectrometry

✍ Jen P. Chang; Douglas E. Kiehl; Allison Kennington 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 144 KB 👁 2 views

Reversed phase high-performance liquid chromatography (RPHPLC) coupled with electrospray ionization mass spectrometry (ESI-MS) was used to separate and characterize the peptides resulting from the tryptic cleavage of somidobove, a recombinant bovine growth hormone. The tryptic digestion of somidobov

Separation of the Sticky Peptides from M

Separation of the Sticky Peptides from Membrane Proteins by High-Performance Liquid Chromatography in a Normal-Phase System

✍ K.A. Lerro; R. Orlando; H.Z. Zhang; P.N.R. Usherwood; K. Nakanishi 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 638 KB

The amphiphilic peptides obtained upon cleavage of membrane proteins, including numerous receptors, are recalcitrant to most separation techniques as a consequence of their limited solubility and tendency to aggregate and adsorb to surfaces. This paper describes HPLC systems that can separate these