Evaluation of reverse transcription loop-mediated isothermal amplification assays for rapid diagnosis of pandemic influenza A/H1N1 2009 virus

✍ Scribed by Mina Nakauchi; Tetsushi Yoshikawa; Hidetaka Nakai; Ken Sugata; Akiko Yoshikawa; Yoshizo Asano; Masaru Ihira; Masato Tashiro; Tsutomu Kageyama

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 76 KB
• Volume: 83
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.21934

✦ Synopsis

Abstract

Two genetic diagnosis systems using reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) technology were evaluated: one for detecting the HA gene of the pandemic influenza A/H1N1 2009 virus (H1pdm RT‐LAMP) and the other for detecting the matrix gene of the influenza A virus (TypeA RT‐LAMP). The competence of these two RT‐LAMP assay kits for the diagnosis of the pandemic influenza A/H1N1 2009 virus was compared using real‐time RT‐PCR assays developed recently on viruses isolated and clinical specimens collected from patients with suspected infection. TypeA RT‐LAMP and H1pdm RT‐LAMP showed almost the same sensitivity as real‐time RT‐PCR for viruses isolated. The sensitivity and specificity of TypeA RT‐LAMP and H1pdm RT‐LAMP were 96.3% and 88.9%, respectively, for clinical specimens. Considering that the ability of the two RT‐LAMP assay kits for detection of the pandemic influenza A/H1N1 2009 virus was comparable to that of the real‐time RT‐PCR assays, and that the assays were completed within 1 hr and did not require any expensive equipment, these two RT‐LAMP assays are promising rapid diagnostic tests for the pandemic influenza A/H1N1 2009 virus at the hospital bedside. J. Med. Virol. 83:10–15, 2011. © 2010 Wiley‐Liss, Inc.