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Evaluation of refolding conditions for a human recombinant fusion cytokine protein, promegapoietin-1a

✍ Scribed by Denis M. Boyle; Gary V. Johnson; Robert A. Heeren; Robert E. Shell; Amit Banerjee; Mark E. Gustafson


Book ID
111709364
Publisher
Portland Press
Year
2008
Tongue
English
Weight
155 KB
Volume
49
Category
Article
ISSN
0885-4513

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## Abstract A concise method was developed for quantifying native disulfide‐bond formation in proteins using isotopically labeled internal standards, which were easily prepared with proteolytic ^18^O‐labeling. As the method has much higher throughput to estimate the amounts of fragments possessing