Evaluation of radiation injury by 1H and 31P NMR of human urine
β Scribed by Victor E. Yushmanov
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 495 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0740-3194
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β¦ Synopsis
Abstract
^1^H and ^31^P NMR techniques were applied to study the changes in metabolite profiles in human urine resulting from radiation exposure following the Chemobyl reactor accident. In cases of acute leukemia and different accumulated doses of external radiation (from 0.20 to 4.00 Sv), the proton spectra were classified on the basis of the peaks due to Nβtrimethyl groups, creatinine, citrate, glycine, and hippurate. Unidentified resonances were observed between 15.9 and 21.4 ppm in six phosphorus spectra of patients with preleukemia and acute leukemia. Characteristic spectral changes were similar for external radiation and Incorporationβinduced internal irradiation. The spectral patterns described may serve as a criterion of radiation injury.
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1H-NMR spectroscopy is a convenient method for determination of diethylcarbamazine (DEC) in urine, and can be used to monitor medication with the drug. Urine samples were mixed with 10% of deuterium oxide as a spectrometer field frequency lock, which is the only sample pretreatment required. Tailore
## Abstract Proton and phosphorus magnetic resonance spectra of substituted methylphosphonic acids have been determined as a function of pH. A method has been developed for measuring the ^31^P shift indirectly by optimal heteronuclear decoupling of the ^1^H spectra of samples and standards. Control
The 13 C, 31 P and 1 H NMR spectra of a series of dioxaphosphinopyridines and pyridoazaphosphinines were studied. The interpretation of the chemical shifts of these compounds was based on model compounds and on selective irradiation and two-dimensional shift correlated NMR techniques.