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Evaluation of quantitative performance of sequential immobilized metal affinity chromatographic enrichment for phosphopeptides

✍ Scribed by Sun, Zeyu; Hamilton, Karyn L.; Reardon, Kenneth F.

Book ID
122409849
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
913 KB
Volume
445
Category
Article
ISSN
0003-2697

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Facile preparation of monolithic immobil
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✍ Liyuan Zhang; Hui Wang; Zhen Liang; Kaiguang Yang; Lihua Zhang; Yukui Zhang πŸ“‚ Article πŸ“… 2011 πŸ› John Wiley and Sons 🌐 English βš– 289 KB

## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the in‐depth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig