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Enrichment of Phosphopeptides Using Biphasic Immobilized Metal Affinity-Reversed Phase Microcolumns

✍ Scribed by Schilling, Michael; Knapp, Daniel R.

Book ID
126471591
Publisher
American Chemical Society
Year
2008
Tongue
English
Weight
425 KB
Volume
7
Category
Article
ISSN
1535-3893

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Facile preparation of monolithic immobil
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## Abstract The selective enrichment of phosphopeptides with good reproducibility is vital for the in‐depth study of the phosphoproteome. Herein, we presented a novel method to prepare monolithic Ti^4+^ or Zr^4+^ immobilized metal affinity chromatography (IMAC) columns. Since succinimide was of hig