Evaluation of pH gradient formation of carrier ampholytes with synthesized isoelectric point markers in capillary isoelectric focusing
β Scribed by Hidesaburo Kobayashi; Mikiyasu Aoki; Miyo Suzuki; Atsushi Yanagisawa; Etsuo Arai
- Book ID
- 108341003
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 954 KB
- Volume
- 772
- Category
- Article
- ISSN
- 1873-3778
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π SIMILAR VOLUMES
## Paris equilibrium iSOeleCtI'k focusing in Cader ampholyte- A modified procedure for the two-dimensional electrophoretic analysis of basic polypeptides is described. This method uses isoelectric focusing with carrier ampholytes in the first dimension, and sodium dodecyl sulfate-electrophoresis i
Rat-incisor phosphoprotein (RIP) has been subjected to isoelectric focusing at 4" in (a) an Ampholine pH gradient of 2.5-4 and (b) an acid pH gradient created by electrolysis of a system of acids and acidic ampholytes and covering the pH range OS-35 In the Ampholine gradient, the RIP unexpectedly fo
In Part I of this series (1)) Jonsson, Pettersson, and Rilbe have described the use of a commercial standard spectrophotometer cuvet (flowthrough cell, type 167-&S, made by Hellma GmbH, Miillheim, Baden, Germany) for density gradient isoelectric focusing of proteins on a microscale. By scanning the