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Evaluation of isotope-coded protein labeling (ICPL) in the quantitative analysis of complex proteomes

✍ Scribed by Alberto Paradela; Miguel Marcilla; Rosana Navajas; Laura Ferreira; Antonio Ramos-Fernandez; Marisol Fernández; Javier Fernando Mariscotti; Francisco García-del Portillo; Juan Pablo Albar

Book ID
116903889
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
389 KB
Volume
80
Category
Article
ISSN
0039-9140

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The combination of isotope coded affinity tag (ICAT) reagents and tandem mass spectrometry constitutes a new method for quantitative proteomics. It involves the site-specific, covalent labeling of proteins with isotopically normal or heavy ICAT reagents, proteolysis of the combined, labeled protein