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Evaluation of inoculum ofCandida guilliermondiigrown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate

✍ Scribed by Débora Danielle Virgínio da Silva; Maria das Graças de Almeida Felipe; Ismael Maciel de Mancilha; Sílvio Silvério da Silva

Book ID
111634566
Publisher
Springer-Verlag
Year
2005
Tongue
English
Weight
197 KB
Volume
121
Category
Article
ISSN
0273-2289

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Effect of glucose:xylose ratio on xylose
Effect of glucose:xylose ratio on xylose reductase and xylitol dehydrogenase activities from Candida guilliermondii in sugarcane bagasse hydrolysate
✍ Débora Danielle Virgínio da Silva; Maria das Graças de Almeida Felipe 📂 Article 📅 2006 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 152 KB

## Abstract The influence of glucose on xylose reductase (XR) and xylitol dehydrogenase (XDH) enzyme activity was evaluated from sugarcane bagasse hydrolysate fermentations with different glucose:xylose ratios (1:25, 1:12, 1:5 and 1:2.5) by employing an inoculum of __Candida guilliermondii__ grown

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To enhance metabolite transfer in the two initial sequential steps of xylose metabolism in yeast, two structural genes of Pichia stipitis, XYL } and XYL " encoding xylose reductase (XR) and xylitol dehydrogenase (XDH), respectively, were fused in frame. Four chimeric genes were constructed, encoding