Evaluation of four methods of DNA distribution data analysis based on bromodeoxyuridine/DNA bivariate data

✍ Scribed by F. Lacombe; F. Belloc; P. Bernard; M. R. Boisseau

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 622 KB
• Volume: 9
• Category: Article
• ISSN: 0196-4763
• DOI: 10.1002/cyto.990090310

✦ Synopsis

Four published methods of DNA-content histogram analysis (those of Fried, Dean and Jett, simplified Dean, and Fox) were compared using a double labeling of different cell populations. Partially synchronized and asynchronous cell populations were incubated with bromodeoxyuridine (BrdUrd) and then stained with an anti-BrdUrd monoclonal antibody and propidium iodide (PI). The fractions of cells in the G1, S , and 6 2 + M phases were calculated by each method and compared with those derived from G1, S, and G2 + M areas plotted on BrdUrdlDNA bivariate histograms, taken as the "true" values. This procedure enabled an optimal choice of method for a given cell population.