A new modification of our detergent technique for the preparation of nuclei for flow cytometric DNA analysis is described. The attainment of low coefficients of variation of the peaks and of quantitative staining of nuclei from different tissues was a problem with the original method. This was solve
A multichannel method for the analysis of DNA content distribution using flow cytometric data
β Scribed by Manabu Takahashi
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 745 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0010-4809
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method is presented for determining ploidy distributions in mouse liver from image analysis with stereological estimations of nuclear size in tissue sections. Nuclear profile distributions obtained from profile measurements were subjected to a mathematical unfolding procedure in order to obtain th
This report describes an immunocytochemical procedure for the simultaneous quantification of bromodeoxyuridine (BrdUrd) incorporated into cellular DNA and total DNA content in individual cells in suspension. Improvement of existing methods was achieved by combining acid denaturation and proteolytic
Flow cytometric analysis was done on exfoliated urothelial cells from 11 control patients and 3 1 patients with transitional cell carcinoma: 9 grade I, 11 grade 11, and 11 grade 111. The determination of aneuploidy by DNA analysis did not provide identification of low-grade tumor cells. Other flow c