✍ Scribed by Christer Hogstrand; Carl Haux
No coin nor oath required. For personal study only.
Two methods to quantify metallothionein (MT), differential pulse polarography (DPP) and radioimmunoassay (RIA), were compared for MT analysis of liver from Zn- and Cd-injected perch (Perca fluviatilis). Nine perch were intraperitoneally injected, twice a week during 2 weeks with ZnSO4 and CdCl2 to yield a total dose of 30 mg Zn and 3 mg Cd per kilogram body weight. Two samples, 100 and 200 mg from each liver, were homogenized separately and further prepared for DPP, RIA, and atomic absorption spectroscopy. MT values obtained by DPP were in good agreement with the MT values determined by RIA (r = 0.92). The relationship between the MT values analyzed with the two methods is described by the formula MTRIA = MTDPP x 0.99-0.048. Analysis of MT was not affected by sample size. MT values from individual liver samples plotted against the Cd and Zn content of the corresponding samples provided a high correlation. The correlation coefficient was 0.86 for MT values obtained by DPP and 0.92 for MT measured by RIA. It is concluded that DPP is a reliable method for analyzing MT in liver.
📜 SIMILAR VOLUMES
A method for the determmatlon of oxalic acid was developed, based on denvatlzatlon wtth o-phenylenedmmme The electroactrve product, dlhydroxyqumoxahne, was determmed by dlfferentlal-pulse polarography and a lmear cahbratlon range from 2 X lo-' to 2 X 10e5 M was obtamed, wrth a detectlon lmut of 5 X