Evaluation of different data-processing options for a flow system with a well-stirred mixing chamber

✍ Scribed by James M. Jordan; Steven H. Hoke; Harry L. Pardue

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 806 KB
• Volume: 272
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80382-u

✦ Synopsis

Ab!stract

This paper describes the evaluation of several data-processmg optIons for a flow-based sample processor with a well-stIrred nuxmg chamber Data-processing options evaluated Include peak-height, peak-area and tune-mterval methods v&h dtierent chorces of reference pomts Results are compared wrth theoret& expectations and effects of expervnental parameters on the different methods are compared by usmg relatwe error coefficients It 1s found that theoretical equations based on a kmetx model for the system descnbe shapes of calibration equations very well and predict slopes of cahbratlon plots to wltlnn a few percent of experunental values These results show that other less ngorous treatments can lead to conclusions that are vahd only for selected sets of circumstances

Keywords Flow system, Cahbratlon plots, Data processmg

There have been many approaches to flowbased sample processors m which unsegmented flow streams were used m conJunctlon with efflclent mung chambers [l-51 A recent vanant of this general approach mvolved mtercalatlon of ahquots of sample mto flowmg streams that flushed them mto muong chambers and subsequently to detectlon systems [6,7] The earher version [61 of this general approach involved parallel flow streams, a small mnnng chamber and relatively fast flow-rates such that fixed ratios of sample and reagent were mixed very rapidly The latter version [7] involved a angle flow stream, a relatively large mxmg chamber and relatively slow flow-rates such that vanable ratios of sample and reagent were mixed