Capillary electrophoresis/time-of-flight mass spectrometry(CE/TOFMS) has been used for analysis of in-gel digests of protein spots excised from two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE). An off-line purification and preconcentration procedure with a Zip
Evaluation of Capillary HPLC/Mass Spectrometry as an Alternative Analysis Method for Gel Electrophoresis of Oligonucleotides
โ Scribed by Jef Rozenski; Karen Vastmans; Arthur Van Aerschot; Piet Herdewijn
- Publisher
- John Wiley and Sons
- Year
- 2004
- Weight
- 59 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0931-7597
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โฆ Synopsis
Abstract
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๐ SIMILAR VOLUMES
A unique preconcentrator was constructed for on-line preconcentration using capillary electrophoresis generating concentration detection limits for peptides in the mid-picomolar range. Several common sheathless electrospray configurations were constructed in conjunction with the preconcentrator and
Two methods for the collection of capillary electrophoresis fractions for analysis by matrix-assisted laser desorption/ionization have been investigated; the first using a novel ion-porous joint design for dropwise fraction collection and the second, a process of electrospray deposition. Sensitivity