✦ LIBER ✦

An on-line preconcentrator and the evaluation of electrospray interfaces for the capillary electrophoresis/mass spectrometry of peptides

✍ Scribed by Christopher J. Herring; Jun Qin

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 96 KB
Volume: 13
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19990115)13:1<1::aid-rcm435>3.0.co;2-a

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✦ Synopsis

A unique preconcentrator was constructed for on-line preconcentration using capillary electrophoresis generating concentration detection limits for peptides in the mid-picomolar range. Several common sheathless electrospray configurations were constructed in conjunction with the preconcentrator and were compared. It was found that a palladium wire interface was the most reliable interface and the problems encountered using this interface are discussed. A tryptic digest of the myosin I heavy chain kinase that contains two phosphopeptides was separated by capillary electrophoresis using this interface and the on-line preconcentrator.

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