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Evaluation of bumetanide as a matrix for prompt fragmentation matrix-assisted laser desorption/ionization and demonstration of prompt fragmentation/post-source decay matrix-assisted laser desorption/ionization mass spectrometry

✍ Scribed by Lavine, Gary; Allison, John

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
153 KB
Volume
34
Category
Article
ISSN
1076-5174

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✦ Synopsis

Bumetanide was evaluated as a matrix for prompt fragmentation matrix-assisted laser desorption/ionization (MALDI) experiments on peptides. Both the MALDI mass spectrum and some physical properties of bumetanide were compared with those of some commonly used matrices. Bumetanide was then evaluated for prompt fragmentation MALDI by comparing the prompt fragmentation produced using bumetanide with that for a-cyano-4-hydroxycinnamic acid and 2,5-dihydroxybenzoic acid. Bumetanide was also evaluated for use in a mixed matrix with a-cyano-4-hydroxycinnamic acid. A 4 : 1 matrix mixture was found to be useful for the post-source decay analysis of a prompt fragment ion. The prompt fragmentation/post-source decay analysis experiment is demonstrated on a prompt fragment ion from the oxidized b-chain of insulin.

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