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Evaluation of bottlenecks in proinsulin secretion by Escherichia coli

✍ Scribed by F.J.M. Mergulhão; M.A. Taipa; J.M.S. Cabral; G.A. Monteiro

Book ID
118471005
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
158 KB
Volume
109
Category
Article
ISSN
0168-1656

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We have determined and monitored the production and release of human proinsulin by genetically engineered Escherichia coli cells. Several M9 media samples were analyzed sequentially after centrifugation with the aid of a rapid automated flow-through thermometric enzyme-linked immunosorbent assay (TE