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Directed evolution to remove expression and secretion bottlenecks of heterologous enterokinase in Escherichia coli

✍ Scribed by Lee, Weiluo; Dalby, Paul

Book ID: 125795171
Publisher: Elsevier
Year: 2014
Tongue: English
Weight: 58 KB
Volume: 31
Category: Article
ISSN: 1871-6784
DOI: 10.1016/j.nbt.2014.05.943

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## Abstract A novel metabolic engineering technique involving the redirection ofcellular carbon fluxes was employed to reduce acetate production in an __Escherichia coli__ culture. Metabolic engineering was achieved by cloning __E. coli__ the gene for the __Bacillus subtilis__ acetolactate synthase