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Functional expression of lipase A from Candida antarctica in Escherichia coli—A prerequisite for high-throughput screening and directed evolution

✍ Scribed by Jan Pfeffer; Monika Rusnak; Carl-Erik Hansen; Rachid Bel Rhlid; Rolf D. Schmid; Steffen C. Maurer

Book ID
113804152
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
320 KB
Volume
45
Category
Article
ISSN
1381-1177

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led