Evaluation of a vancomycin chiral stationary phase in packed capillary supercritical fluid chromatography

✍ Scribed by Jürgen Dönnecke; Lars A. Svensson; Olle Gyllenhaal; Karl-Erik Karlsson; Anders Karlsson; Jörgen Vessman

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 273 KB
• Volume: 11
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1999)11:7<521::aid-mcs4>3.0.co;2-n

✦ Synopsis

Vancomycin immobilized on an aldehyde functionalized silica support in packed capillary columns has been used as a chiral stationary phase in supercritical fluid chromatography under subcritical conditions. The use of carbon dioxide with methanol as the mobile phase modifier is shown to offer an extended scope of chiral separations compared with reversed-phase liquid chromatography, especially for basic analytes. It could be demonstrated that the chiral recognition of vancomycin for different analytes is based on several kinds of interactions. Large differences in enantioselectivity in a homologous series of local anesthetic compounds indicate that the fit of a host᎐guest interaction between an analyte and vancomycin could be very precise. Nonlinear van't Hoff plots implied that the enantiomers of metoprolol and the modifiers evaporated from their adsorption sites at different temperatures.