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Evaluation of a real-time polymerase chain reaction assay for the detection of Dientamoeba fragilis

โœ Scribed by Adriana Calderaro; Chiara Gorrini; Sara Montecchini; Simona Peruzzi; Giovanna Piccolo; Sabina Rossi; Franco Gargiulo; Nino Manca; Giuseppe Dettori; Carlo Chezzi

Book ID
116408883
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
156 KB
Volume
67
Category
Article
ISSN
0732-8893

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Molecular Beacons: A Real-Time Polymeras
Molecular Beacons: A Real-Time Polymerase Chain Reaction Assay for Detecting Salmonella
โœ Wilfred Chen; Grisselle Martinez; Ashok Mulchandani ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 146 KB

Molecular beacons are oligonucleotide probes that become fluorescent upon hybridization. We developed a real-time PCR assay to detect the presence of Salmonella species using these fluorogenic reporter molecules. A 122-base-pair section of the himA was used as the amplification target. Molecular bea