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Evaluation of a modified rapid staining protocol for human sperm morphology assessment

✍ Scribed by M. Lanham; J.E. Swain


Book ID
116480934
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
55 KB
Volume
98
Category
Article
ISSN
1556-5653

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## Abstract A triple‐stain technique has been developed to score normal acrosome‐reacted human sperm in fixed smears. Live and dead sperm are first differentiated using the vital stain trypan blue. Sperm are then fixed in glutar‐aldehyde, dried onto slides, and the postacrosomal region and acrosome