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Sperm decondensation and semen parameters: utilization of a simple staining technique for the evaluation of human sperm decondensation

✍ Scribed by M.-L. Windt; P. M. de Beer; D. R. Franken; J. Rhemrev; R. Menkveld; C.J. Lombard; T. F. Kruger


Book ID
115137414
Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
493 KB
Volume
26
Category
Article
ISSN
0303-4569

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A triple-stain technique for evaluating
✍ Talbot, Prudence ;Chacon, Richard S. 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 572 KB

## Abstract A triple‐stain technique has been developed to score normal acrosome‐reacted human sperm in fixed smears. Live and dead sperm are first differentiated using the vital stain trypan blue. Sperm are then fixed in glutar‐aldehyde, dried onto slides, and the postacrosomal region and acrosome