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Evaluation of 5′-MGB hybridization probes for detection of Bordetella pertussis and Bordetella parapertussis using different real-time PCR instruments

✍ Scribed by Irina Afonina; Mark Metcalf; Alan Mills; Walt Mahoney

Book ID: 116408342
Publisher: Elsevier Science
Year: 2008
Tongue: English
Weight: 126 KB
Volume: 60
Category: Article
ISSN: 0732-8893
DOI: 10.1016/j.diagmicrobio.2007.10.019

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## Abstract A polymerase chain reaction (PCR) assay in real‐time for detection of __B. pertussis__ using SYBR green I as the reporter fluorophore and LightCycler instrument (a thermocycler coupled to a fluorescence detection device) was established and evaluated. The amplified amplicon using series