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Evaluation and comparison of therapeutic monitoring of whole-blood levels of cyclosporin A and its metabolites in renal transplantation by HPLC and RIA methods

✍ Scribed by Kristian Šafarčı́k; Hana Brozmanová; Vladimı́r Bartoš; Alexandr Jegorov; Milan Grundmann

Book ID: 117074345
Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 130 KB
Volume: 310
Category: Article
ISSN: 0009-8981
DOI: 10.1016/s0009-8981(01)00570-8

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## Abstract A LC‐MS/MS method was developed and validated for the determination of cyclosporine A (CsA) and its three phase 1 metabolites AM1, AM9, and AM4N in whole blood and lymphocytes isolated on the Histopaque gradient. 200 μL of whole blood was precipitated with 10 mol/L zinc sulfate in aceto