European functional analysis network (EUROFAN) and the functional analysis of the Saccharomyces cerevisiae genome (minireview)
โ Scribed by Dr. Bernard Dujon
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 907 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0173-0835
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The function of the open reading frame (ORF) YOR108w of Saccharomyces cerevisiae has been analysed. The deletion of this ORF from chromosome XV did not give an identifiable phenotype. A mutant in which both ORF YOR108w and LEU4 gene have been deleted proved to be leucine auxotrophic and alpha-isopro
Using PCR-ligated long flanking homology cassettes, null alleles of six open reading frames (ORFs) from chromosome II have been created in Saccharomyces cerevisiae. Deletants were constructed in three genetic backgrounds: FY1679, W303 and CEN.PK2. Tetrad analysis of heterozygous deletants revealed t
Six open reading frames (ORFs) of unknown function from Saccharomyces cerevisiae chromosome XV, three from the left and three from the right arm, were deleted in two diploid strains by the short flanking homology method (Wach et al., 1994). Transformants were selected as Geneticin (G418)-resistant c