A specially designed apparatus and conditions are described for the rapid analysis of ribosomal proteins by two-dimensional gel electrophoresis on a micro scale. The resolution of proteins in electropherograms is comparable to that obtained with other systems, but because of miniaturization, only 0.
Eukaryotic Ribosomal Proteins : Interspecific and Intraspecific Comparisons by Two-Dimensional Polyacrylamide-Gel Electrophoresis
β Scribed by Jean Delaunay; Claude Mathieu; Georges Schapira
- Book ID
- 115113403
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 586 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1432-1327
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The 80S ribosome of Schizosaccharomyces pombe contains 93 proteins as determined by twodimensional electrophoresis on polyacrylamide gels. Of these, 76 are basic and 17 are acidic at pH 8.7. 38 proteins could be assigned unambiguously to the large sub-unit and 19 to the small. 67 proteins were extra