Ethidium bromide fluorescence in living and fixed cells
β Scribed by J. C. Stockert
- Book ID
- 104829954
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 587 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0028-1042
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π SIMILAR VOLUMES
The measurement of DNA in tissue samples fixed in ethanol/acetic acid is described. Small, fixed tissue samples are digested by warm alkaline treatment followed by neutralization with HCl, and DNA is determined by complex formation with the dye ethidium bromide (EB). When standard DNA from calf thym
Until now the fluorescence assay with ethidium bromide has only been used on pure DNA. This assay depends on the difference in fluorescence between single-and double-stranded D N A (dsDNA). Cross-links in D N A are measured by the return of fluorescence of dsDNA after heat denaturation at PH 12. Und