✍ Scribed by W. Schmidt; Th. Scheper; K. Schügerl
No coin nor oath required. For personal study only.
Ethanol was produced with Zymomonas mobilis Z6 (ATCC 29191), in batch culture with synthetic medium on glucose as substrate and in the presence of aspartate. The concentrations of glucose, phosphate, ammonium, ethanol and dissolved 0 2 and CO 2 in the medium and 02 and CO2 in the outlet gas as well as the cell mass by culture fluorescence were measured on-line. Cell mass, glucose and aspartate concentrations were measured off-line.
In the presence of a sufficient amount of aspartate, the ethanol inhibition effect can be reduced considerably. However, the improvement with yeast extract is more incisive. The relationship between the intensity of culture fluorescence and cell mass concentration is linear, if sufficient aspartate is present.
List of symbols
kg/m 3 CTR kg/(m 3 9 h) N, NH 4 kg/m 3 P kg/m 3 P % PO 4 kg/m 3 QE kg/(kg-h) QN, NH~ kg/(kg" h) Qv kg/(kg, h) Qs kg/(kg, h) S kg/m ~ S o kg/m ~ Yx/s kg/kg /~ h -~ aspartate concentration CO 2 transfer rate nitrogen concentration from NH2 product (ethanol) concentration product (ethanol) yield phosphate concentration specific ethanol production rate specific nitrogen uptake rate from NH~specific phosphate uptake rate specific substrate (glucose) uptake rate glucose concentration initial glucose concentration yield coefficient specific growth rate
📜 SIMILAR VOLUMES
The performance of ethanolic bacterium, Zymomonas mobilis, ATCC 31821, cross-linked to glutaraldehyde-treated, gelatin-coated spherical glass beads in an immobilized cell reactor (ICR) was evaluated for feed glucose concentrations of 1SO and 200 g 1-l. A synthetic medium, containing inorganic nitrog