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Estimation of tyrosine by the attenuation of 4-iodophenol enhanced chemiluminescence

โœ Scribed by Candy, Thomas E. G. ;Mantle, David ;Jones, Peter


Publisher
John Wiley and Sons
Year
1991
Weight
345 KB
Volume
6
Category
Article
ISSN
0884-3996

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โœฆ Synopsis


Abstract

Tyrosine markedly attenuates the chemiluminescence output intensity from the 4โ€iodophenol enhanced chemiluminescence assay system in a manner consistent with competition between the amino acid and luminol for the 4โ€iodophenoxy radical. This effect provides the basis for a sensitive assay of tyrosine. Interference by the other amino acids has been assessed; major interference by cysteine can be removed by incubation with iodoacetic acid.


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