The intensity of 4-I-phenol-enhanced chemiluminescence from the luminol-H,O,-horseradish peroxidase system is markedly attenuated in the presence of low concentrations of non-enhancer phenols. Under the conditions studied, the effect is not associated with competition between 4-I-phenol and non-enha
Estimation of tyrosine by the attenuation of 4-iodophenol enhanced chemiluminescence
โ Scribed by Candy, Thomas E. G. ;Mantle, David ;Jones, Peter
- Publisher
- John Wiley and Sons
- Year
- 1991
- Weight
- 345 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0884-3996
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โฆ Synopsis
Abstract
Tyrosine markedly attenuates the chemiluminescence output intensity from the 4โiodophenol enhanced chemiluminescence assay system in a manner consistent with competition between the amino acid and luminol for the 4โiodophenoxy radical. This effect provides the basis for a sensitive assay of tyrosine. Interference by the other amino acids has been assessed; major interference by cysteine can be removed by incubation with iodoacetic acid.
๐ SIMILAR VOLUMES
The enhanced chemiluminescence reaction (ECL) was applied to the study of horseradish peroxidase (HRP) inactivation during the oxidation of p-iodophenol. Enzyme inactivation was shown to be the main reason for light decay in the course of the reaction. No individual effect of luminol and p-iodopheno
Light is emitted in systems containing N-chloramines or hypochlorite, H(2)O(2) and N-(4-aminobutyl)-N-ethylisoluminol (ABEI). The emission is enhanced by 4-iodophenol (PIP) in alkaline solution (1 mol/L NaOH), while at lower pH range (9-11) PIP is not only inactive but also its presence reduces chem